Molecular cloning and analysis of the endogenous retrovirus chemically induced from RFM/Un mouse cell cultures.

Characterization of a molecular clone of RFM/Un mouse chromosomal DNA that contains a full-length endogenous murine leukaemia virus-related proviral genome.

A 12.4 kbp HindIII chromosomal DNA fragment harbouring an apparently intact 9.2 kbp endogenous murine leukaemia virus (MuLV)-related proviral genome was isolated from an RFM/Un strain mouse by molecular cloning and designated pRFM #6. Nucleotide sequence analysis revealed the following characteristic features in the pRFM #6 provirus: a distinct 200 bp sequence in the long terminal repeat (LTR) ...

P-128: The Effect of DNA Methyl Transferase1 Inhibitor (RG108) on DNA Methylation Pattern of Cloned Mouse Embryos

Background: In somatic cell nuclear transfer (SCNT) method of cloning, transferred nucleus should be dedifferentiated from differentiated state to embryonic state. Molecular analysis showed that the reprogramming in the transferred nucleus was incomplete and cloned embryos have epigenetic abnormalities such as high DNA methylations levels. Since methylation in pre-implantation embryos has a cri...

Jaagsiekte Sheep Retrovirus from That of the Highly Related Exogenous of Sheep Reveal a Different Cell Tropism of Three Type D Endogenous Retroviruses Molecular Cloning and Functional Analysis

P-86: Production of Cloned Mice by Somaticm Cell Nuclear Transfer

Background: For several years, mammalian cloning by splitting an early embryo or nuclear transfer into oocytes method has been successfully performed. Cloning is now also possible using adult somatic cells. Although it has now been 15 years since the first cloned mammals were generated from somatic cells using nuclear transfer (NT), the success rate for producing live offspring by cloning is lo...

Cloning and Expression of Coxsakievirus B3 Viral Protein-1 in E. Coli

Viral protein-1 (VP1) is a major capsid protein of Coxsakievirus B3 (CVB3) that plays an important role in directing viruses towards permissive cells and acts as a main antigenic site of the virus in eliciting of host immune response, hence it seems VP1 can be considered as a vaccine candidate against CVB3 infection. In this study, cDNA of VP1 was prepared, cloned into pET expression vector and...